optimized root production during micropropagation of new iranian apple hybrid rootstock (az x m9): effects of fe-eddha and thiamine

in vitro propagation of az x m9 new apple rootstock resulted of breeding program of vegetative apple rootstock in iran was investigated. nodal explants were inoculated in murashige and skoog (ms) medium supplemented with various concentrations of 6-benzylaminon purine (bap) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (naa) (0.1 mg/l). we examined the effect of different concentrations of iron sequestrene (fe-eddha) and thiamin vitamin in ½ ms and linsmaier and skoog (ls) media supplemented with indol-3-butric acid (iba) (1 mg/l) on rooting of attained plantlets. according to our results, 0.4 bap + 0.1 naa treatment in ms medium was the best component which resulted in the highest proliferation rate (6.73). as well, we estimated the highest root number in ½ ms + 0.15 g/l fe-eddha + 2.4 g/l thiamin.